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Cell culture is one of the most important tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis. It is also used in drug screening and development, and large scale manufacturing of biological compounds (e.g., vaccines, therapeutic proteins). The major advantage of using cell culture for any of these applications is the consistency and reproducibility of results that can be obtained from using a batch of clonal cells.

The biotechnology international industry is a horizontal discipline that combines extensive scientific and technological knowledge and involves the use of various techniques like: recombinant DNA, bioprocesses, cell and tissue culture, etc. in the intervention to improve production and achieve the solution of problems associated with products and processes of multiple productive sectors: as agriculture, food,

Protein purification can be a costly and time-consuming process in the manufacture of biomolecules. Either from a tissue or by their overexpression in a model organism, e.g. bacteria, yeast, or mammalian cells in culture, one of the objectives of the process is to obtain the highest functional protein yield while minimizing contaminants. Many factors can